Coding
Part:BBa_K2926067
Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
Fusion protein of Opy2 (yeast), mCherry and pVIII (bacteriophage M13) with purification tag
The extracellular cysteine-rich domain of the membrane protein Opy2 from S. cerevisiae was n-terminally fused to the red fluorescing reporter protein mCherry. Additionally, the M13 bacteriophage major coat protein pVIII was fused to the C-terminus of mCherry. To enable easy protein purification a hexahistidine tag was fused to the C-terminus of the protein.
Sequence and Features
Sequence was validated by Sanger sequencing.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |